International Journal of Medical Sciences (IJMS)

Escherichia coli are regarded as the most important causes of intestinal illnesses in the world and also a significant public health challenge. Intestinal sicknesses which are caused by E. coli infection are often under diagnosed while doing usual microbiological analysis, particularly in resource constrained settings. However, the use of molecular tests could be helpful to identify the distribution of intestinal illnesses of E. coli infection and also its clinical importance. The distribution analysis of virulence factors which exist among various E. coli pathotypes which include Shiga toxin-producing E. coli (STEC) will offer substantial insights with regard to the mechanisms through which various E. coli strains lead to diseases and result in the growth of different E. coli types. This present investigation therefore is an attempt to evaluate the incidence of intestinal illnesses of E. coli in clinical samples taken from patients. The samples from 150 patients and 15 controls were collected and then screened for E. coli. Compatible colonies taken from 150 individuals (150 patients and 15 controls) were characterized through performing biochemical test, a set of Polymerase Chain Reaction (PCR) for recognition of six virulence factors. The clinical samples from patients were within two months -two year attending. Pyelonephritis were associated by pili (pap gene, hemolysin (hly gene), aerobactin (aer gene), translocon pore-forming subunit espB and Shiga toxins (Stx1 and Stx2) respectively. They were related with six intestinal illnesses pathotypes. The incidence of hlyA and papC (both 40%) was highest, and was followed by espB, aer, stx1, stx2(30%,25%,20% and 18%) respectively. Although, how and whether these mixtures of genes affect their pathogenicity is an issue that have to be investigated and elucidated. The findings obtained in this study highlight the significance of epidemiological and microbiological surveillance of STECs. It also determines if the development of control measures will diminish the risks which are associated with intestinal illnesses from E.coli.